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Formal Lab Report 1: Growth and Enumeration of Escherichia coli

this report is based on lab representation 2 and 3data sets have been attachedvisual and data numberThe readings from the spectrophotometer include the transmittance and/ absorbance values that have been provided. These are being plotted in the required graphs.The following are what you will need for your lab report:Semi log Graph of CFU/mL (y-axis) vs Time (x-axis) to use for calculationsSemi log Graph of CFU/mL (y-axis) vs % Transmittance (x-axis) to use as the Standard Growth Curve of E. coli for Lab 3 Enumeration via Turbidimetric EstimationCalculation of the number of generations at time tCalculation of the mean growth rate of E. coliCalculation of the generation time of E. coliCalculation of the CFU/mL for each method of enumeration (table)The data table can be found under “Data for Growth of E. Coli F2020 ” you need to use this in addition to your assigned dataset to complete the lab report.The dataset includes the experiment to which each of these images relate. You will then used these images and results to tackle that specific experiment/ calculations.Attached is a PPT which shows the steps for calculating the Number of Generations, Mean Growth Rate of Bacteria and generation time.Remember to use the Exponential (Log) Phase for these calculations.When completing the visual counts remember that you only consider plates with more than 30 and fewer than 300 (>30 and <300) CFUs i.e. statistically significant. The two images are plates that are replicates of the same dilution.Also remember that when using duplicate or triplicate plating from the same dilution, take the average of the plate counts and then proceed with the calculations.In the Dataset (3.1 POUR PLATES), to calculate the final dilution factor (FDF) you need to take the reciprocal of the dilution that was given.NO TITLE PAGE NEEDEDSTART FROM ABSTRACT