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variation in cold tolerance in Drosophila

A scientific lab report based one of the lab. I have attached instruction in doc, please follow the rubric, thanks.I also attached the link about how the lab is demonstrated (for intro and method purpose) please watch them, thanksthe data is in the excel, please use the figure inside.in the discussion part, a “t-test” needs to be included, please calculate the variance (just enter measured values).*referencing style from the Journal of Experimental Biology!* (link is provided)link for a demonstration of wetlab: https://www.youtube.com/watch?v=NikP6_FjpTM